KMID : 0369819910210030143
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Jorunal of Korean Pharmaceutical Sciences 1991 Volume.21 No. 3 p.143 ~ p.147
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Immobilization of ¥á- amylase on Polyethylene Glycol Grafted Polyurethane Film
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±è¼ºÈ£/Kim SH
ÇÏÁ¤Çå/Ha CH
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Abstract
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¥á-amylase was, immobilized on the surface of polyethylene glycol(M.W. 2000) grafted polyurethane film using diisocyanate in an attemp to develop enzyme immobilized polymeric materials. The surface morphology of the modified polyurethane film was examined by SEM. Effects of pH and temperature on the activity of the immobilized ¥á-amylase were investigated. The optimal pH range of the activity was 7.0¡7.5. The immobilized ¥á-amylase demonstrated high thermal stability and maintained consistent activity during long-term storage.
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KEYWORD
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